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Frequently Asked Questions

CADET BRAF MUTATION DETECTION ASSAY

Transitional cell carcinoma (TCC), also known as urothelial carcinoma (UC), is the most common cancer of the canine urinary tract. TCC/UC accounts for an estimated 1-2% of all cancer cases diagnosed in dogs, with an anticipated 80,000+ diagnosed cases this year.

TCC/UC affects the bladder, urethra, and kidneys of male and female dogs and also the prostate of males. It is most often detected in the trigone of the bladder, a triangular region of smooth mucosa inside the dorsal wall of the neck of the bladder. Advancing TCC/UC often results in straining to urinate, repeated frequent attempts to urinate, blood in the urine, and bacterial infection. Thickening of the bladder wall can lead to partial or complete obstruction of urine entering the bladder from the ureters, which may lead to kidney failure.

While any breed is susceptible to developing TCC/UC, these breeds have higher than average incidence rates: American Eskimo Dog, Australian Cattle Dog, Australian Shepherd, Beagle, Bichon Frise, Border Collie, Parson Russell Terrier, Lhasa Apso, Rat Terrier, Russell Terrier, Scottish Terrier, Shetland Sheepdog, West Highland White Terrier, and Wire Fox Terrier. When combined, these breeds account for over a third of all diagnosed TCC/UC cases in purebred dogs.

Use a clean household container to collect urine from your dog. We suggest you use a single-use, disposable container, cup or tray; however a Tupperware or ladle or similar container is suitable as long as it is well sanitized between uses, especially if you are collecting samples from different dogs. This is to ensure that urine from one dog does not contaminate that of another dog.


Diagnosis/monitoring service packs are shipped to veterinary professionals as either a 1, 3 or 6 pack. Custom-sized orders are also available on request. For each urine sample to be submitted for testing a service pack comprises,

  1. (1) small (3”x3”x3”) cardboard box that contains one (1) 60ml capacity urine collection pot with screw top lid, inside a small snap-seal plastic bag. The collection pot contains a small volume of a proprietary preservative liquid that must not be discarded. The collection pot also has a sticker on the side to show you precisely how much urine to add.
  2. (1) envelope containing

    1. a paper copy of the instructions for collection and return shipping of the urine specimen to our laboratory
    2. a submission form – Part A must be completed and we encourage you to complete Part B
    3. a FedEx Clinical Pak onto which has been placed an EXEMPT ANIMAL SPECIMEN sticker
    4. a return pre-paid FedEx shipping label

Your service pack for the annual subscription will be sent to you by the AKC and includes:

  1. (3) Urine Sample Collection Service Packs in small (3”x3”x3”) cardboard boxes. The lids of these boxes have been left open for your convenience. One of these packs will be used to return the urine sample collected from your dog every 4-months over the next year.
  2. (1) Envelope containing a paper copy of the instructions
  3. (3) sample submission forms/questionnaires
  4. (3) return pre-paid FedEx shipping labels
  5. Each small white box contains one (1) 60ml capacity urine collection pot with screw top lid, inside a small snap-seal plastic bag. The collection pot contains a small volume of a preservative liquid that must not be discarded. The collection pot also has a sticker on the side to show you precisely how much urine to add.
  6. (1) A second small snap seal bag containing an absorbent pad, FOR SHIPPING THE URINE SAMPLE BACK TO THE TESTING LABORATORY
  7. (1) FedEx Clinical Pak for return shipping, onto which has been placed an EXEMPT ANIMAL SPECIMEN sticker.


CADET BRAFSM Mutation Detection diagnostic and monitoring service packs will be sent to you by Sentinel Biomedical. These pack contents are the same as above, except you will be sent either 1 set, 3 sets, or 6 sets of all collection materials.


Here are the steps you should follow to collect and ship urine samples to the testing laboratory:

  1. Remove one Urine Sample Collection Service Pack from the outer box (if a subscription pack), or from your FedEx envelope (if a diagnostic/monitoring service pack).
  2. Take the urine collection pot out of the small plastic bag. Do not discard the preservative, or the absorbent pad supplied

    with the pot (a

    second bag and pad supplied for return shipping).

  3. Using a permanent marker, write the first and last name of your dog, and the date of collection, on the label on the outside of the sample pot.
  4. Use a clean (preferably disposable) household container to collect urine from your dog.  Examples include paper or plastic food trays or cups.
  5. Remove the screw cap from the urine collection pot and pour the urine carefully into the sample pot containing the preservative. Add a sufficient volume of urine to reach the \’fill to here\’ line. If necessary, set the pot aside and top up with additional urine later on in the day. It is not necessary to refrigerate the urine.
  6. Replace the screw-cap lid tightly and swirl to mix the urine with the preservative.
  7. Write the time of collection on the outside of the urine collection pot.
  8. Once the pot lid is screwed on tightly, place the collection pot inside the supplied plastic snap-seal bag containing the absorbent pad.
  9. Close the snap-seal plastic bag and place the sealed bag back inside the small white cardboard sample box.
  10. Close the lid of the small  box securely and place the box inside the supplied FedEx Clinical Pak. PLEASE DO NOT WRITE ON BOX.
  11. Complete the sample submission/questionnaire form and place inside the FedEx Clinical Pak with the sample box. NOTE: If the submission form provided has a white top copy and a yellow carbon copy, tear off the top (white) copy and send back to the laboratory in the FedEx Clinical Pak. Keep the yellow copy for your record.
  12. Seal the FedEx Clinical Pak, peel off the return sticker and attach the label to the front of the FedEx Clinical Pak.
  13. Drop off the Clinical Pak at any FedEx authorized ShipCenter. Please note that samples cannot be shipped using FedEx drop boxes
  14. PLEASE send e-mail to: CADETINBOUND@SentinelBiomedical.com with the FedEx tracking number from your return label included on the the subject header.

Canine bladder cancer (TCC/UC) is generally a disease of mid-to- late life, with 95% of cases occurring in dogs age 6 years and older. Canine bladder cancer is rarely diagnosed until the disease is at an advanced stage. Detecting bladder cancer sooner gives dog owners and veterinarians more time to develop a treatment plan.